Team:TU-Eindhoven/Protocols

Protocols

Below, the protocols we’ve used during our labwork are listed. They can be downloaded in pdf format.

General protocols

Preparation general necessities

Streaking glycerol stock

Agarose gel electrophoresis

Cloning and BioBricking

PCR Amplification

Vector linearization

Gibson assembly

Gel purification

Transformation and protein expression

Transformation into competent cells

Colony picking and Colony PCR

Sequencing at BaseClear

Small culturing

Project specific protocols

Hydrogel seeding experiments

Dextran hydrogel synthesis

Sample preparation for scanning electron microscopy

Lysostaphin microbial activity assay